Lipophilicity (log P), is a physicochemical property of drugs having an application in new drug discovery and bioavailability studies. To reduce the time consumption and cost of log P determination by shake flask method, RP-HPLC method was presented for determination of lipophilicity of antiviral drugs (emtricitabine, nevirapine and zidovudine) using Agilent TC-C18 (4.5 x 250 mm, 5 μm particle size) column. The mobile phase consisted of a mixture of methanol and phosphate buffer (pH 7.4) saturated with octanol in isocratic elution mode. The capacity factor (k) for the drugs was determined by their retention time (Rt). The determined log P of the drugs emtricitabine, nevirapine and zidovudine was found to be -1.574, 1.457 and 0.417 respectively. The result by RP-HPLC method was nearly similar to the virtual log P calculated values. This method could be used for log P determination for neutral and basic antiviral drugs in regular analysis.
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